Modulation of the GABA_A Receptor by Srif in Rat Thalamus

Abstract

Somatostatin has been reported to modulate GABA_A receptor complex in many brain structures. This study was conducted to investigate somatostatin modulation of the GABA_A receptor binding in several rat diencephalic structures, focusing primarily on the thalamus, as this structure plays important roles. Animals were assigned to control conditions. Changes in specific binding of GABA_A receptor as labelled with [³⁵S]t-Butylbicyclophosphorothionate (TBPS) were assessed by in vitro quantitative autoradiography with the aid of a computer assisted image analysis system. Our results reveal the presence of higher densities in several thalamic structures located principally in the part of thalamus. We demonstrate for the first time the presence of a modulatory effect of somatostatin on the GABA_A receptor complex in this brain region in rats. Indeed, the peptide affected in a concentration-dependent manner; the binding of [³⁵S]-Tertiary Butylbicyclophosphorothionate (TBPS) to the convulsant site of the GABA_A receptor complex in thalamic structures with an affinity in the micromolar range (10^-3 to 3.10^-6M). The inhibitory effect of somatostatin is observed in all thalamic structures analyzed. The absence of a specific region effect of somatostatin on the binding of [³⁵S]-TBPS, suggests the presence of a homogenous subunit GABA_A receptor composition. Furthermore, GABA and muscimol, a GABA_A receptor agonist, enhanced the affinity of somatostatin effect on [³⁵S]-TBPS. This suggests that somatostatin allosterically modifies [³⁵S]-TBPS binding through a mechanism similar to that of GABA.