Polyherbal EMSA ERITIN Blocks Nuclear Factor-Kappa B (NF-κB) and Proinflammatory Cytokines in Irradiated Mice

Abstract

Radiation therapy is commonly used in cancer treatment that produce deleterious effects, such as normal cell death (apoptotic or necrotic) and suppression of hematopoietic stem cells. This effect was caused by ion rays that can rise to high of free radical. The high of free radical trigger the inflammation of cells. EMSA ERITIN is medicinal herb used as preventative agents or radioprotective to reduce the deleterious effects of radiation. EMSA ERITIN contains soy, red rice and coconut water extract. This study aimed to analyze the effect of polyherbal EMSA ERITIN to the expression of transcription factor of inflammation (NF-κB). We also observe the proinflammatory cytokines (TNF-α and IFN-γ) in BALB/c mice after radiation. Polyherbal EMSA ERITIN grouped into three doses: Low dose (1.04 mg g^-1 BW), optimum dose (3.125 mg g^-1 BW) and high dose (9.375 mg g^-1 BW). The radiation dose that applied to mice is 5 Gy. The oral administration of EMSA ERITIN started 24 h after radiation and continued until 2 weeks. Data were analyzed using One-way ANOVA (p<0.05) and Tukey test using SPSS 16.0 for Windows. The result showed that the level of NF-κB, TNF-α and IFN-γ in irradiated mice significantly decreased when the mice received EMSA ERITIN. All dose of polyherbal EMSA ERITIN showed more effective than EPO (Hemapo Epoetin alfaâ™) treatment to reduced TNF-α, IFN-γ and NF-κB. So that, EMSA ERITIN is one of potential herbal medicine act as a preventive agent or radioprotective after radiation.