Optimization of Microwave-Assisted Extraction of a Bioherbicide from Canavalia ensiformis Leaves
- 1 University of São Paulo, Brazil
- 2 Institute of Chemistry of São Carlos, Brazil
Abstract
The objective of this study was to optimize the operational conditions of the Microwave-Assisted Extraction (MAE) of Canavalia ensiformis leaves by Response Surface Methodology (RSM), based on analysis of phenolic compounds obtained from the aqueous extracts. Optimal conditions for MAE were temperature of 100°C, extraction time of 10 min and ramp of temperature of 5 min, using approximately 2 g of dry plant material and 30 mL of water. The extracts obtained were determined by HPLC-UV. Among the phenolic compounds present in plant leaves, ferulic acid was selected as reference in all analyzes of the extracts. The results showed that the developed model was able to describe the effects and the relationship between them in the performance of the extraction, taking full advantage of all the limitations of the equipment.
DOI: https://doi.org/10.3844/ajessp.2016.27.32
Copyright: © 2016 Darlan Ferreira Silva, Eduardo Bessa Azevedo and Maria Olímpia Oliveira Rezende. This is an open access article distributed under the terms of the
Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Keywords
- Bioherbicide
- Microwave-Assisted Extraction
- Optimization
- Canavalia ensiformis
- Response Surface Methodology