Potency of Pepsin Soluble Collagen from Indonesian Local Goat Skin as an Antioxidant

Abstract

Pepsin Soluble Collagen (PSC) hydrolyses is derived from the collagen extraction process from Indonesia local goat skin (Kacang) was carried out in some previous studies. This study is considering the first research used the collagen from the goat skin as an antioxidant compound. The main purpose of study were to investigate antioxidant activity of PSC hydrolysis after pepsin treatment. The experiment was applied enzymatic treatment using pepsin as 0.1 U unit/g per g collagen for various (0, 30, 60, 90 and 120 min) hydrolysis time under 37°C to determine the degree of hydrolysis. Collagen solubility, protein molecular weight and value of radical scavenging activities were observed. The result of the study showed that the highest of degree of hydrolysis of PSC from goat skin found at 90 min incubation (20.05±0.76%). The highest collagen solubility of PSC was hydrolyzed for 30 min with the value 2.59 mg/mL. PSC before hydrolysis has molecular weight of 57.82 - 162.06 kDa and after hydrolysis using pepsin and incubation at 37ºC for 120 min, the molecular weight decreased into 9.09 - 46.75 kDa. The concentration of 500 ppm of PSC after hydrolysis for 90 min has 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) radical scavenging activity 59.35% with IC50 198.59 ppm. This study indicated that hydrolysis time influenced solubility, molecular weight and radical scavenging activity of PSC hydrolysate from Indonesia local goat skin.